1st Faculty of Medicine Charles University 1st Faculty of Medicine Charles University BIOCEV
lvysa 08.02.2021

Open Position - PhD student - Dráber Lab

Open PhD position in Draber lab - Cellular signaling in autoimmune diseases

Project description:

Immune system has evolved to protect the body from invading pathogens and cancer. Yet aberrant inflammation can lead to severe autoimmune diseases. In order to properly regulate activation and termination of the immune responses, individual cells must communicate with each other and provide the information about ongoing inflammation. One means of communication is the production of small soluble proteins termed cytokines, that are detected by specific receptors on target cells. Proper sensing of these cytokines allow immune cells to activate adequate immune reaction. On the other hand, erroneous response to the cytokine stimulation can lead to severe autoimmunity. The present research aims to uncover new molecular mechanisms how crucial pro-inflammatory cytokines signal and identify new possible venues to modulate their activity in the case of autoimmunity.

The project will involve study of protein complexes via mass-spectrometry, preparation of knockout cell lines via CRISPR/Cas9 approach, and analysis of their signaling using methods of molecular biology. Data obtained from cell lines will be further studied in vivo using mouse models and murine knockout strains. The research project will be carried at BIOCEV research campus in Vestec. We offer monthly salary 20.000 CZK which is supplemented with stipend 15.000 CZK and five weeks of annual paid leave.

Candidate profile:

Candidate should be deeply interested in science, proficient in English, willing to collaborate and share experience with colleagues, and willing to work with murine models. The candidate should hold a master degree in immunology, cell biology, biochemistry, molecular biology, or a related field. Prior laboratory experience is advantageous. Candidates interested in this research should contact Peter Draber directly at email: peter.draber@lf1.cuni.cz

Suggested readings:

Systematic analysis of the IL-17 receptor signalosome reveals a robust regulatory feedback loop. EMBO J. 2020 Sep 1;39(17):e104202. doi: 10.15252/embj.2019104202. Epub 2020 Jul 21.

TBK1 and IKKε prevent TNF-induced cell death by RIPK1 phosphorylation. Nat Cell Biol. 2018 Dec;20(12):1389-1399. doi: 10.1038/s41556-018-0229-6.

 

lvysa 17.12.2020

Open Position - PhD student - Hons Lab

The Leukocyte Motility Lab of the First Faculty of Medicine, Charles University located in BIOCEV seeks motivated PhD students to join our newly established research team.

Project

Immunity depends on leukocyte ability to rapidly migrate within lymphoid organs and peripheral tissues. We will work at the interface between cell biology and immunology and study how leukocytes distinguish various environmental cues and interpret them in their migratory behavior. Our primary focus are mechanical aspects – we want to understand how leukocytes recognize physical stress, overcome obstacles or navigate in topologically complex environments. To this end, we will use microfabrication and live cell imaging.

For more insight please see:

• https://www.biocev.eu/en/research-program/cellular-biology-and-virology.4/leukocyte-motility.66

• Cellular locomotion using environmental topography. Nature. 2020 Jun;582(7813):582-585.

• Chemokines and integrins independently tune actin flow and substrate friction during intranodal migration of T cells. Nat Immunol. 2018 Jun;19(6):606-616.

We are looking for dedicated candidates interested in cell biology, cell migration, cytoskeleton, immunology and microscopy.

Applicants are expected to apply for a doctoral program at the Faculty of Science at Charles University by the end of December 2020. See:

https://www.natur.cuni.cz/eng/study/applicants/ph.d.-study

Positions are open from January 2021.

We offer an opportunity to work in a newly established research group within the BIOCEV with its exceptional core resources and equipment.

Contact

Applicants are encouraged to provide a brief CV with summary of recent work to Miroslav Hons, PhD(miroslav.hons@lf1.cuni.cz)


lvysa 17.12.2020

Group Leader - Virology : COVID-19

We are seeking a motivated and independent TOP-NOTCH scientist that is capable of a) forming new research group, b) recruit & lead PhD students effectively, c) apply & receive domestic and international funding, and d) publish in respected journals: for a position of Junior Principal Investigator.

We seek primarily those with at least 2-year international research experience at respected US- or EU-based University or Academic Institution with excellent skills in scientific writting and research design.

An excellent track record of research accomplishments is our primary aim! The candidates are expected to participate on a set of research-oriented discussion meetings and present their ideas clearly.

Research areas that involve pathobiology and pathogenesis of Coronavirus disease (COVID – 19).

We offer a three-year extendable contract, with the salary scale based on performance. Our labs are located in a modern science building and offer superb research facilities. Research groups enjoy a free access to a broad portfolio of scientific core facilities, including a mouse clinic, microscopy, cytometry, mass spectrometry and many others.

The successful candidate will be selected by a collective decision of the committee upon realization of at least two meetings that include the scientific presentation and, if successful, another meeting focused on the lab planning and grant proposals.

For further details, please see https://biocev.lf1.cuni.cz/ or https://www.biocev.eu/en.

Contact

Applicants are encouraged to provide a CV in English and a short list of professional goals to lucie.vysatova@lf1.cuni.cz

lvysa 15.07.2020

Group Leader

We are seeking a motivated and independent TOP-NOTCH scientist that is capable of a) forming new research group, b) recruit & lead PhD students effectively, c) apply & receive domestic and international funding, and d) publish in respected journals: for a position of Junior Principal Investigator.

We seek primarily those with at least 2-year international research experience at respected US- or EU-based University or Academic Institution with excellent skills in scientific writting and research design.

An excellent track record of research accomplishments is our primary aim! The candidates are expected to participate on a set of research-oriented discussion meetings and present their ideas clearly.

Research areas that involve the following topics are especially welcome i.e. therapy resistance in solid oncology, gene therapy, novel therapies in cancer, circulating tumor cells, and approaches to combine global data such as application of machine learning in cancer therapy.

We offer a three-year extendable contract, with the salary scale based on performance. Our labs are located in a modern science building and offer superb research facilities. Research groups enjoy a free access to a broad portfolio of scientific core facilities, including a mouse clinic, microscopy, cytometry, mass spectrometry and many others.

The successful candidate will be selected by a collective decision of the committee upon realization of at least two meetings that include the scientific presentation and, if successful, another meeting focused on the lab planning and grant proposals.

For further details, please see https://biocev.lf1.cuni.cz/ or https://www.biocev.eu/en.

Contact

Applicants are encouraged to provide a CV in English and a short list of professional goals to lucie.vysatova@lf1.cuni.cz

lvysa 10.07.2019

Research position available - Stopka Lab

http://stopka-lab.lf1.cuni.cz

The lab seeks researcher position (either postdoc or PhD student) in molecular aspects of normal and malignant hematopoiesis. We offer deep expertise in the transgenic models, gene expression, flow cytometry, microscopy and tumor cell-next generation sequencing.

We are looking for open-minded person with solid training from previous lab. Welcome is an expertise in molecular and cellular biology, genetics, and/or biochemistry. We offer a salary scale based on performance and two-year extendable contract. Our lab is located in a modern science building and offers superb research facilities.

Project: Molecular mechanisms in Myelodysplastic syndrome (MDS)and Acute Myeloid Leukemia (AML). Previous work of our lab has elucidated how hematologic malignancies such as MDS or AML prevent progression upon effects of inhibition in DNA methylation by Azacitidine (AZA) or alterantively, develop resistence while acquiring additional somatic mutations in genes involved in transcriptional repression. The applicant is expected to be involved in evaluating functionality of epigenetic pathways in order to provide experimental evidence of how tumor cells can bypass therapeutic resistance.

The position is currently OPEN.

Contact

Applicatns are encouraged to provide english-written CV by email to tstopka@lf1.cuni.cz