1st Faculty of Medicine Charles University 1st Faculty of Medicine Charles University BIOCEV
tstopka 14.06.2018

PHEO/PGL PROTEOME

THE FIRST ON-LINE DRAFT OF HUMAN PHEOCHROMOCYTOMA/PARAGANGLIOMA PROTEOME

Pheochromocytoma and paraganglioma (PHEO/PGL) are rare neuroendocrine tumors arising from chromaffin cells of adrenal medulla and from extra-adrenal sympathetic­ and parasympathetic ganaglia, respectively. Low incidence (0.8 per 100 000) classifies PHEO/PGL among rare diseases. The majority of PHEO/PGL are benign, yet show high morbidity and mortality due to excessive production of catecholamines, leading to hypertension, arrhythmia and stroke. Up to 25 percent are malignant. Therapy for patients with metastatic PHEO/PGL is limited with 5 year survival often less than 50 percent. 

Pilot quantitative analysis of human pheochormocytoma and paraganglioma sample (5797 proteins).  LC-MS/MS analysis of 3 individual tumor samples. We used iTRAQ labeling with 4 labels and analyzed 2 different PHEO and 1 sympathetic PGL sample provided by our partner (Karel Pacak, NICHD-NIH, Bethesda, MD). Healthy adrenal medulla was used as a control . We identified over 5700 proteins/protein groups with FDR 0.01. Of those 4400 were identified with 2 or more unique peptides, each with at least 0,95% confidence.
´

number of views: 244 last modification: tstopka, 14.06.2018
Page ranking: If you think the article is not up-to-date, click here.